tgradient thermal cycler (Biometra)
86
Structured Review
Biometra
tgradient thermal cycler
Tgradient Thermal Cycler, supplied by Biometra, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgradient+cycler/cycler+tgradient+thermal/pm41384813-75-7-10
Average 86 stars, based on 1 article reviews
Tgradient Thermal Cycler, supplied by Biometra, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgradient+cycler/cycler+tgradient+thermal/pm41384813-75-7-10
Average 86 stars, based on 1 article reviews
tgradient thermal cycler - by Bioz Stars,
2026-10
86/100 stars
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DNA Amplification:Article Title: Thioesterase-mediated side chain transesterification generates potent Gq signaling inhibitor FR900359 Article Snippet: Thermal cycling was performed in a Biometra TGradient cycler with an initial denaturation step at 98 °C for 30 s, followed by 30 to 33 cycles of DNA denaturation at 98 °C for 10 s, primer annealing for 20 s and DNA amplification at 72 °C for 30 s/kb amplified, and a final extension step at 72 °C for 2 min. Optimal annealing temperature for specific primer combinations was determined experimentally using temperature gradients from 46–64 °C. .. Thermal cycling was performed in a Amplification:Article Title: Thioesterase-mediated side chain transesterification generates potent Gq signaling inhibitor FR900359 Article Snippet: Thermal cycling was performed in a Biometra TGradient cycler with an initial denaturation step at 98 °C for 30 s, followed by 30 to 33 cycles of DNA denaturation at 98 °C for 10 s, primer annealing for 20 s and DNA amplification at 72 °C for 30 s/kb amplified, and a final extension step at 72 °C for 2 min. Optimal annealing temperature for specific primer combinations was determined experimentally using temperature gradients from 46–64 °C. .. Thermal cycling was performed in a Article Title: Self-assembled micelles based on N-octyl-N'-phthalyl-O-phosphoryl chitosan derivative as an effective oral carrier of paclitaxel. Article Snippet: Herein, we describe a novel amphipathic chitosan derivative (N-octyl-N’-phthalyl-O-phosphoryl chitosan, abbreviated as OPPC) as an effective oral delivery platform for P-gp substrates, especially paclitaxel (PTX).. OPPC could readily self-assemble into micelles, solubilize and encapsulate PTX into the hydrophobic inner core of OPPC with superior loading capacity to chitosan.. PTX/OPPC micelles possessed improved intestinal epithelial permeability and oral bioavailability of PTX evaluated by in situ perfusion and pharmacokinetic studies. Reverse Transcription:Article Title: Effect of Perilla frutescens Extracts on Porcine Jejunal Epithelial Cells. Article Snippet: Green-leaved Perilla frutescens extracts were investigated on their effect on cell proliferation of the porcine jejunal epithelial cell line, IPEC-J2, as well as on the gene expression of cell cycle or cancer-related genes.. Some extracted compounds were, however, susceptible to degradation in cell culture medium, whereas others were found to be stable during the entire experimental time.. Control experiments also included the assessment of H2O2 generation in cell culture medium caused by oxidation of natural extract compounds, which was proved to be absent at low extract concentrations. other:Article Title: PCR melting profile (PCR MP) - a new tool for differentiation of Candida albicans strains Article Snippet: Reproducibility of the PCR MP was tested on ten optionally chosen C. albicans isolates (group 2 of strains) and using batches of DNA from two separate DNA extractions amplified in parallel assays and obtained using two extraction methods (Genomic Mini AX Yeast kit - A&A Biotechnology, Poland or GeneMATRIX Yeast DNA Purification kit - Eurx, Poland), employing two different Polymerase Chain Reaction:Article Title: Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris Article Snippet: All oligonucleotides were synthesized by MWG Eurofins. .. The polymerase chain reaction (PCR) was performed in a Article Title: Self-assembled micelles based on N-octyl-N'-phthalyl-O-phosphoryl chitosan derivative as an effective oral carrier of paclitaxel. Article Snippet: Herein, we describe a novel amphipathic chitosan derivative (N-octyl-N’-phthalyl-O-phosphoryl chitosan, abbreviated as OPPC) as an effective oral delivery platform for P-gp substrates, especially paclitaxel (PTX).. OPPC could readily self-assemble into micelles, solubilize and encapsulate PTX into the hydrophobic inner core of OPPC with superior loading capacity to chitosan.. PTX/OPPC micelles possessed improved intestinal epithelial permeability and oral bioavailability of PTX evaluated by in situ perfusion and pharmacokinetic studies. Article Title: Whale lice ( Isocyamus deltobranchium & Isocyamus delphinii ; Cyamidae) prevalence in odontocetes off the German and Dutch coasts – morphological and molecular characterization and health implications Article Snippet: .. Polymerase chain reactions (PCR) were performed in a total volume of 50 μl, comprising 25 μl of MyTaq Red Mix, 2x (Bioline GmbH, Germany), 1 μl each of primers at 20 μM, 1 μl of DNA template and 22 μl of DEPC-H 2 O in a |